Temporary

Part:BBa_J70550:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-15)

{15,18;13,10} BsaXI BioScaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
    Illegal AgeI site found at 896
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

designed

after created, then test with

Forward (J70550-test-f): CGGCCGCTTCTAGAGTACTAGTAGCGGC

Reverse (J70550-test-r): CGATGATCATGAGATCTTCGCCGGCCGT

Source

designed from J04450 using

Forward primer (J70550-f): gtttcttcgaattcgcggccgcttctagagCTCcaatacgcaaaccgcctctccc

Reverse primer (J70550-r): cttcctgcagcggccgctactagtaCCTGTCCTCCtataaacgcagaaaggcccacccga

References